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BOOK EXAM - Molecular Biology of the Cell

Answer 3 out of the 4 questions. Recommended length - 600 words/question

May 2020

1. You are interested in finding molecular interaction partners of protein X that is highly expressed in rat brain. Provide the basic principles of 2 different methods for identifying proteins binding to protein X. How do you evaluate the specificity of identified interactions?

2. Protein kinases and their significance in cell signaling

3. Define: a. CRISPR b. Tight junctions c. CryoEM d. RNA interference e. Golgi apparatus f. Cytokinesis

4. DNA Repair

Exams from other years

1. You have identified a protein and its gene. The protein is highly expressed in the nervous system and certain peripheral tissues of mice. You are interested in finding biological functions of this protein. Explain at least three experimental approaches that can ve used for this purpose. What information they provide.

2. Research articles on the origin of life put importance on the 'RNA world'. What do you mean by this concept? Provide evidence that supports the 'RNA' world in early evolution.

3. What Kind of molecules are protein kinases? How do they work and regulate? Live examples of its role in biological processes…

4. Short note: a. CRISPR b. miRNA c. Proteasome d. CryoEM e. Telomerase f. RNA sea


1. DNA Repair

2. Protein kinases and their significance in cell signaling

3. Protein-protein interactions. Describe a method that can be used in live cell imaging…

4. Define: a. CRISP b. Standard free energy change c. Golgi apparatus d. Proteasome e. Telomesare f. CryoEM


1. How and why are ions transported across the biological membrane?

2. You are interested in finding molecular interaction partners of protein X that is highly expressed in rat brain. Provide the basic principles of 3 different methods for identifying proteins binding to protein X. How do you evaluate the specificity of identified interactions?

3. Telomers and telomerases

4. a. There is no motor protein specific for intermediate filaments. Provide a plausible explanation for this finding, considering the structure of different classes od skeletal elements. b. Muscle contraction consumes a lot of ATP. Name and briefly describe the two major underlying ATP-dependent mechanisms.


1. RNA splicing

2. Define: a. CRISPR b. Protein mass spectrometry c. Standard free energy change d. RNA interference e. Proteasome f. Telomerase/telomers

3. Mechanisms of ribosomal protein synthesis

4. How are ions transported across biological membranes?