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gene_regulation_in_eukaryotes - Tenttiwiki
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Gene regulation in eukaryotes

1.6.2011

Answer to three questions

  1. Describe briefly the main roles of the C-terminal domain (CTD) in the largest subunit of RNA POL II in transcription and 5' capping of RNA
  2. Describe briefly the role of the RITS complex in formation of heterochromatin
  3. Draw (or describe) two hypothetical models explaining how two different transcription factors can act synergistically to enhance assembly of the initiation complex for RNA POL II
  4. Describe three different ways to regulate gene expression post-transcriptionally ?? (en vastannut tähän joten en muista tarkalleen)

17.5.2011

Answer to three of the following questions

  1. Describe briefly the structure and roles of TFIIH in initiation of transcription.
  2. Describe briefly the sequence of events (starting from production of pri-miRNAs) which lead to miRNA mediated gene silencing through inhibition of translation in animals.
  3. Describe briefly a mechanism (either real or developed by your own imagination) by which a single transcription factor can act both as a negative and a positive refulator of transcription.
  4. Characteristic features of histone 3 in silent chromatin are general lack of acetyl groups and methylation of K9. It is also well known that modifications of amino acids in H3 are often mutually exclusive. Picture below is showing the inhibitory effect of midifications on other modifications in H3.

Assume that H3 is currently modified in the following way: mK4, aK9, aK14, aK18, mK27, pS28. (m=methylation, a=acetylation, p=phsophorylation.) The possible modification enzymes are: methyl transferases, acetylases, deacetylases, protein kinases and protein phosphatases. What is the order of events that generate modifications leading to chromatin inactivation?

25.5.2010

Answer to three questions

  1. Describe briefly the structure and roles of TFIIH in initiation of transcription
  2. Draw (or describe) two hypothetical models explaining how two different transcription factors can act synergistically to enhance assembly of the initiation complex for RNA POL II
  3. Describe briefly the role of the RITS complex in formation of heterochromatin
  4. Describe three different DNA binding domains in transcritpion factors

11.5.2010

Answer to three of the following questions

  1. Describe briefly the main roles of the C-terminal domain (CTD) in the largest subunit of RNA POL II in transcription and RNA processing
  2. Describe briefly how miRNAs are generated from pri-miRNAs in mammalian cells
  3. The same question as the last one in the 16.12.2004 exam
  4. Describe the structure and DNA binding of TFs containing a homeodomain

28.5.2009

Answer to three of the following questions

  1. Describe briefly the structure of TFIIH and how this factor participates in initiation of transcription
  2. Draw (or describe) two hypothetical models explaining how two different transcription factors can act synergistically to enhance assembly of the initiation complex for RNA POL II
  3. Describe briefly the sequence of events (starting from production of pri-miRNAs) which leads to miRNA mediated gene silencing both through target mRNA degradation and inhibition of translation in animals
  4. Describe three different ways to regulate gene expression through endogenous antisense transcripts

14.5.2009

Answer to three of the following questions

  1. Describe briefly the main roles of the C-terminal domain (CTD) in the largest subunit of RNA POL II in transcription and RNA processing
  2. Describe briefly a mechanism (either real or developed by your own imagination) by which a single transcription factor can act both as a negative and a positive regulator of transcription
  3. Describe briefly how miRNAs are generated from pri-miRNAs in mammalian cells
  4. The same question as the last one in the 16.12.2004 exam

28.5.2008

Answer to three of the following questions

  1. Describe briefly the structure of TFIIH and how this factor participates in initiation of transcription
  2. Describe briefly the molecular events leading to activation of heat shock genes
  3. Describe briefly the sequence of events (starting from production of pri-miRNAs) which leads to miRNA mediated gene silencing both through target mRNA degradation and inhibition of translation in animals and plants
  4. Describe the structure and DNA binding of TFs containing a homeodomain

11.1.2005

Answer to three of the following questions

  1. Describe briefly the structure and roles of TFIIH in initiation of transcription. Kuvaa lyhyesti TFIIH:n rakenne ja sen osuus traskription aloituksessa.
  2. Draw (or describe) two hypothetical models explaining how two different transcription factors can act synergistically to enhance assembly of the initiation complex for RNA POL II. Piirrä (tai kuvaa) miten kaksi eri transkriptiofaktoria voi toimia synergisesti tehostaessaan RNA POL II:n initiaatiokompleksin muodostumista.
  3. Describe briefly the sequence of events (starting from production of pri-miRNAs) which leads to miRNA mediated gene silencing both through target mRNA degradiation and inhibition of translation in animal cells. Kuvaa lyhyesti tapahtumaketju (aloittaen pri-miRNA:n tuotosta), joka johtaa miRNA-välitteiseen geenien hiljentämiseen kohde-mRNA:n degradaation tai translaation inhibition kautta eläinsolussa.
  4. Describe the structure and DNA binding of TFs containing a homeodomain. Kuvaa homeodomainin omaavien TF:n rakenne ja DNA:han sitoutuminen.

16.12.2004

Answer three of the following questions

  1. Describe briefly the main roles of the C-terminal domain (CTD) in the largest subunit of RNA POL II in transcription and RNA processing
  2. Draw (or describe) two hypothetical models explaining how two different transcription factors can act synergistically to enhance assembly of the initiation complex for RNA POL II.
  3. Describe briefly how miRNAs are generated from pri-miRNAs
  4. Characteristic features of histone 3 in silent chromatin are general lack of acetyl groups and methylation of K9. It is also well known that modifications of amino acids in H3 are often mutually exclusive. Below you find a picture showing the effect of existing modifications on other potential modifications in H3. (Blocked line means inhibition.)

Assume that H3 is currently modified in the following way: mK4, aK9, aK14, aK18, mK27 and pS28 (m = methylation, a = acetylation, p = phosphorylation). The possible modification enzymes are: methyl transferases, acetylases, deacetylases, protein kinases and protein phosphatases. What is the order of events that creates modifications leading to chromatin inactivation?